Turning a list into variables in python

This question already has an answer here:

In Python, how do I read a file line-by-line into a list? 35 answers

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Use a context manager:

with open(myfile, 'r') as f:
    for line in f:
        print(line)

Not sure how your file is formatted, but if it is in the form of:

Question
Answer
Question
Answer
.
.
.
Question N
Answer N

You can call enumerate() over a generator within the context manager and go to specific lines. For example, if you know the line number or perhaps build a dictionary of Q&A for even lines:

with open(myfile, 'r') as f:
    q_a = {}
    for num, line in enumerate(line for line in f):
        if num % 2 = 0:
            question = line
        else:
            q_a[question] = line

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